Detection and partial molecular characterization of Grapevine fleck virus, Grapevine virus D, Grapevine leafroll-associated virus -5 and -6 infecting grapevines in Brazil
نویسندگان
چکیده
منابع مشابه
Grapevine leafroll-associated virus 3
Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if intervention strategies are not implemented. Several viruses from the family...
متن کاملElisa detection of grapevine fleck virus ( GFkV )
Résumé — Détection par Elisa du virus de la marbrure de la vigne (GFkV = grapevine fleck virus). L’association du Grapevine fleck virus (GFkV) avec les symptômes de la marbrure de la vigne a été établie par Elisa. La détection par Elisa du GFkV dans les plants de vigne n’est pas possible pendant la totalité du cycle végétatif : dans nos conditions, en Alsace, les mois de juin et juillet c...
متن کاملOccurrence of Grapevine Leafroll-Associated Virus Complex in Napa Valley
Grapevine leafroll disease (GLD) is caused by a complex of several virus species (grapevine leafroll-associated viruses, GLRaV) in the family Closteroviridae. Because of its increasing importance, it is critical to determine which species of GLRaV is predominant in each region where this disease is occurring. A structured sampling design, utilizing a combination of RT-PCR based testing and sequ...
متن کاملComplete nucleotide sequence and genome organization of Grapevine fleck virus.
The complete nucleotide sequence of Grapevine fleck virus (GFkV) genomic RNA was determined. The genome is 7564 nt in size, excluding the 3'-terminal poly(A) tail, is characterized by an extremely high cytosine content (ca. 50%), and contains four putative open reading frames and untranslated regions of 291 and 35 nt at the 5' and 3' ends, respectively. ORF 1 potentially encodes a 215.4 kDa pol...
متن کاملPartial Genome Organization, Identification of the Coat Protein Gene, and Detection of Grapevine leafroll-associated virus-5.
ABSTRACT The genome of Grapevine leafroll-associated virus-5 (GLRaV-5) was cloned, and the sequence of 4766 nt was determined. Degenerate oligonucleotide primers designed from the conserved closterovirus heat shock 70 protein (HSP 70) homologue were used to obtain viral-specific sequences to anchor the cloning of the viral RNA with a genomic walking approach. The partial nucleotide (nt) sequenc...
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ژورنال
عنوان ژورنال: Ciência Rural
سال: 2012
ISSN: 1678-4596
DOI: 10.1590/s0103-84782012005000119